Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys
نویسندگان
چکیده
منابع مشابه
PURIFICATION AND PARTIAL CHARACTERIZATION OF A COAGULANT SERINE PROTEASE FROM THE VENOM OF THE IRANIAN SNAKE Agkistrodon halys
Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sepharose and high performance liquid ch...
متن کاملPurification and Partial Characterization of a Thrombin-Like Enzyme (AH144) from Venom of Iranian Snake Agkistrodon Halys
The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...
متن کاملpurification and partial characterization of a thrombin-like enzyme (ah144) from venom of iranian snake agkistrodon halys
the snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. purification and partial characterization of a thrombin-like enzyme from the venom of the iranian snake, agkistrodon halys, was the aim of this study. purification was carried out by a combination of variety of chromatographic methods that included: gel fil...
متن کاملPartial Purification and Characterization of Anticoagulant Factor from the Snake (Echis carinatus) Venom
Objective(s): Snake venoms contain complex mixture of proteins with biological activities. Some of these proteins affect blood coagulation and platelet function in different ways. Snake venom toxin may serve as a starting material for drug design to combat several pathophysiological problems such as cardiovascular disorders. In the present study, purification of anticoagulation facto...
متن کاملCloning and characterization of Adinbitor, a novel disintegrin from the snake venom of Agkistrodon halys brevicaudus stejneger.
Adinbitor was cloned from Agkistrodon halys brevicaudus stejneger and characterized as a novel disintegrin. In this study, total RNA was extracted from venom gland and used in RT-PCR to generate a cDNA which is 219 bp long. The sequence encoded a polypeptide composed of 73 amino acids, including 12 cysteines, an RGD motif, and the signature motif of disintergrin. Recombinant Adinbitor (rAdinbit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Venomous Animals and Toxins including Tropical Diseases
سال: 2009
ISSN: 1678-9199
DOI: 10.1590/s1678-91992009000300005